LOCUS       HAS3-pGEc2              6885 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6363..6492)
                     /note="3'-ITR"
     misc_feature    complement(6061..6275)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5513..6054
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4692..4714
                     /note="attB2"
     misc_feature    5492..5497
                     /note="BstB1 site"
     misc_feature    4715..5410
                     /note="Super GFP"
     misc_feature    5411..5461
                     /note="AviTag"
     misc_feature    5462..5485
                     /note="His Tag"
     PCR_frame       3011..4691
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..4669
                     /note=" OPTIMIZED HAS3 coding region"
     CDS             4670..4691
                     /note="TEV site"
                     /translation="ENLYFQG"
     vector          join(4700..6885,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4699
                     /source="HAS3 pDONR"
                     /type="Entry Clone"
     source          1..6885
                     /dnas_title="HAS3-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGCCTGTGC AGCTGACAAC AGCTCTGAGA GTCGTGGGCA CATCTCTGTT
     3061 TGCTCTGGCC GTGCTGGGAG GAATTCTGGC CGCCTATGTG ACCGGCTACC AGTTCATCCA
     3121 CACCGAGAAG CACTACCTGA GCTTTGGCCT GTACGGCGCC ATTCTGGGAC TGCATCTGCT
     3181 GATCCAGAGC CTGTTCGCCT TCCTGGAACA CAGAAGAATG AGAAGGGCCG GACAGGCCCT
     3241 GAAGCTGCCA TCTCCTAGAA GAGGATCCGT GGCCCTGTGT ATTGCCGCCT ACCAGGAAGA
     3301 TCCCGACTAC CTGCGGAAGT GTCTGAGAAG CGCCCAGAGA ATCAGCTTCC CCGACCTGAA
     3361 AGTGGTCATG GTGGTGGACG GCAACAGACA GGAGGACGCC TACATGCTGG ACATCTTTCA
     3421 CGAAGTGCTG GGCGGCACAG AGCAGGCCGG ATTTTTTGTG TGGCGGAGCA ATTTTCACGA
     3481 GGCCGGCGAA GGCGAGACTG AGGCTTCTCT GCAGGAAGGG ATGGACAGAG TCAGGGATGT
     3541 CGTGCGGGCC AGCACCTTCA GCTGTATCAT GCAGAAATGG GGCGGCAAGC GGGAAGTGAT
     3601 GTACACCGCC TTTAAGGCCC TGGGCGACAG CGTGGACTAT ATCCAGGTCT GCGATAGCGA
     3661 CACAGTGCTG GATCCTGCCT GCACCATCGA GATGCTGAGA GTGCTCGAAG AGGACCCACA
     3721 GGTCGGAGGT GTCGGAGGGG ATGTGCAGAT CCTGAACAAA TACGACAGCT GGATCTCCTT
     3781 CCTGTCCTCC GTGCGGTACT GGATGGCCTT CAATGTGGAA AGAGCCTGCC AGAGCTACTT
     3841 CGGCTGCGTG CAGTGTATCT CTGGCCCCCT GGGCATGTAC AGAAACAGCC TGCTCCAGCA
     3901 GTTTCTGGAA GATTGGTATC ACCAGAAGTT TCTCGGCAGC AAGTGCAGCT TCGGCGACGA
     3961 CAGACACCTG ACCAATAGAG TGCTGAGCCT GGGCTACCGG ACCAAGTACA CAGCCAGATC
     4021 CAAGTGCCTG ACCGAGACAC CCACAAAGTA CCTGAGATGG CTGAACCAGC AGACCCGGTG
     4081 GTCCAAGTCC TACTTCAGAG AGTGGCTGTA CAACTCCCTG TGGTTCCACA AGCACCACCT
     4141 CTGGATGACC TACGAGAGCG TCGTGACCGG ATTCTTCCCC TTCTTTCTGA TCGCCACCGT
     4201 GATTCAGCTC TTCTACCGGG GCAGAATCTG GAATATCCTG CTGTTCCTGC TGACCGTGCA
     4261 GCTCGTGGGA ATCATCAAGG CCACCTACGC CTGTTTTCTG CGGGGCAACG CCGAGATGAT
     4321 CTTCATGAGC CTGTACTCCC TGCTGTATAT GTCTAGCCTG CTGCCCGCCA AGATCTTCGC
     4381 CATTGCCACA ATCAACAAGA GCGGCTGGGG CACCAGCGGC AGAAAGACCA TCGTGGTCAA
     4441 CTTCATCGGC CTGATCCCCG TGTCTATCTG GGTGGCAGTG CTGCTCGGAG GACTGGCCTA
     4501 TACAGCCTAC TGCCAGGACC TGTTTAGCGA AACCGAGCTG GCCTTTCTGG TGTCTGGCGC
     4561 CATCCTGTAT GGCTGCTATT GGGTCGCCCT GCTGATGCTG TACCTGGCCA TCATTGCCAG
     4621 AAGATGCGGC AAGAAGCCCG AGCAGTATAG CCTGGCTTTT GCCGAGGTCg aaaacttgta
     4681 ctttcaaggc tCCAGCTTTC TTGTACAAAG TGGTATGAGC AAGGGCGAGG AACTGTTCAC
     4741 CGGCGTGGTG CCCATCCTGG TGGAACTGGA TGGCGACGTG AACGGCCACA AGTTCTCTGT
     4801 GCGGGGAGAG GGCGAAGGCG ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     4861 CACCGGCAAG CTGCCCGTGC CTTGGCCTAC CCTCGTGACC ACACTGACCT ACGGCGTGCA
     4921 GTGCTTCAGC AGATACCCCG ACCATATGAA GCGGCACGAC TTCTTCAAGA GCGCCATGCC
     4981 CGAGGGCTAC GTGCAGGAAC GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG
     5041 AGCCGAAGTG AAGTTCGAGG GCGACACCCT CGTGAACCGG ATCGAGCTGA AGGGCATCGA
     5101 TTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAACTTCA ACAGCCACAA
     5161 CGTGTACATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA
     5221 CAACGTGGAA GATGGCAGCG TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     5281 AGATGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA
     5341 GGACCCCAAC GAGAAGCGGG ACCACATGGT GCTGCTGGAA TTCGTGACCG CCGCTGGCAT
     5401 CACCCACGGC ATGAGCGGCC TGAACGACAT CTTCGAGGCC CAGAAAATCG AGTGGCACGA
     5461 GCACCACCAT CATCACCATC ACCACTGATG ATTCGAAAAA TAATAAGGAT CCAATCAACC
     5521 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC
     5581 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT
     5641 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT
     5701 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG
     5761 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC
     5821 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC
     5881 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT
     5941 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC
     6001 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT
     6061 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC
     6121 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG
     6181 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG
     6241 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT
     6301 TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA
     6361 CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA
     6421 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA
     6481 GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC
     6541 AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG
     6601 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     6661 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC
     6721 TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT
     6781 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA
     6841 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG
//

